Karakterisasi Mikrostruktur dan Komposisi Elemental Whey Protein sebagai Bahan Film Biodegradable
Main Article Content
Abstract
Akumulasi limbah plastik yang menimbulkan berbagai permasalahan lingkungan mendorong pengembangan bahan kemasan pangan ramah lingkungan, salah satunya melalui penelitian penggunaan whey protein sebagai bahan dasar pembuatan film biodegradable. Penelitian ini bertujuan untuk menganalisis mikrostruktur dan komposisi unsur whey protein menggunakan teknik Scanning Electron Microscopy (SEM) dan Energy Dispersive X-ray Spectroscopy (EDS), serta mengevaluasi kaitannya dengan potensi aplikasi whey protein sebagai bahan film biodegradable. Serangkaian pengamatan mikrostruktur dilakukan menggunakan mikroskop elektron pemindai (SEM), sedangkan analisis komposisi unsur dilakukan melalui spektroskopi sinar-X dispersi energi (EDS) untuk mengetahui profil kandungan unsur pada permukaan whey protein. Data dianalisis secara deskriptif untuk menginterpretasikan sifat morfologi dan kimia dari sampel. Hasil SEM menunjukkan bahwa whey protein memiliki struktur permukaan yang kasar, tidak beraturan, dan cenderung membentuk agregat yang ditandai dengan pori-pori mikroskopis. Sifat-sifat ini mendukung luas permukaan yang tinggi dan reaktivitas yang baik, meskipun agregasi yang besar dapat berdampak negatif terhadap stabilitas dan homogenitas larutan. Hasil EDS menunjukkan dominasi unsur karbon dan oksigen, serta keberadaan mineral seperti natrium (Na), kalium (K), kalsium (Ca), dan magnesium (Mg). Mineral-mineral ini diketahui berperan dalam pembentukan jaringan dan stabilitas struktur protein melalui ikatan ionik. Kombinasi antara mikrostruktur yang kompleks dan komposisi unsur fungsional menjadikan whey protein sebagai bahan yang berpotensi untuk dikembangkan menjadi film biodegradable yang mendukung inovasi kemasan pangan berkelanjutan.
Article Details

This work is licensed under a Creative Commons Attribution 4.0 International License.
References
Carpena, M., Nuñez-Estevez, B., Soria-Lopez, A., Garcia-Oliveira, P., & Prieto, M. A. (2021). Essential oils and their application on active packaging systems: A review. Resources, 10 (1), 7.
Chen, H., Wang, J., Cheng, Y., Wang, C., Liu, H., Bian, H., Pan, Y., Sun, J., & Han, W. (2019). Application of protein-based films and coatings for food packaging: A review. Polymers, 11(12), 2039.
Chen, Y., Wang, J., Xu, L., Nie, Y., Ye, Y., Qian, J., Liu, F., & Zhang, L. (2024). Effects of different plasticizers on the structure, physical properties and film forming performance of curdlan edible films. Foods, 13 (23), 3930.
Chowdhury, S. N., Nag, S., Ashish, & Tripathi, K. M. (2017). Recent advances in bio-polymers for innovative food packaging. In Mishra, A.K., Hussain, C.M., & Mishra, S.B., editor. Biopolymers: Structure, Performance and Applications. New York: Nova Science Publishers
de Castro, R. J. S., Domingues, M. A. F., Ohara, A., Okuro, P. K., dos Santos, J. G., Brexó, R. P., & Sato, H. H. (2017). Whey protein as a key component in food systems: Physicochemical properties, production technologies and applications. Food Structure, 14.
Fahrullah, F., Noersidiq, A., Kisworo, D., & Maruddin, F. (2024). Evaluating physicochemical properties of whey-chia seed edible films for biodegradable packaging. Tropical Animal Science Journal, 47(4), 519–528.
Fahrullah, F., Noersidiq, A., & Maruddin, F. (2022). Effects of glycerol plasticizer on physical characteristic of whey-konjac films enriched with clove essential oil. Journal of Food Quality and Hazards Control, 9, 226–233.
Fahrullah, F., Radiati, L. E., Purwadi, P., & Rosyidi, D. (2020). The Effect of different plasticizers on the characteristics of whey composite edible film. Jurnal Ilmu dan Teknologi Hasil Ternak, 15(1), 31-37.
Gerna, S., D’Incecco, P., Limbo, S., Sindaco, M., & Pellegrino, L. (2023). Strategies for exploiting milk protein properties in making films and coatings for food packaging: A Review. Foods, 12(6), 1271.
Guerrero, P., & de la Caba, K. (2017). Protein-based films and coatings. Edible Food Packaging: Materials and Processing Technologies. Boca Raton: CRC Press.
Handayani, R., & Nurzanah, H. (2018). Karakteristik edible film pati talas dengan penambahan antimikroba dari minyak atsiri lengkuas. Jurnal Kompetensi Teknik, 10(1), 1-11.
Janjarasskul, T., & Tananuwong, K. (2019). Role of whey proteins in food packaging. In Smithers, G., editor. Reference Module in Food Science. Elsevier.
Kumar, A., Hasan, M., Mangaraj, S., M, P., Verma, D. K., & Srivastav, P. P. (2022). Trends in edible packaging films and its prospective future in food: A Review. Applied Food Research, 2 (1), 100118.
Maruddin, F., Ratmawati, R., Fahrullah, F., & Taufik, M. (2018). Karakterisitik edible film berbahan whey dangke dengan penambahan karagenan. Jurnal Veteriner, 19 (2), 291-297.
Minj, S., & Anand, S. (2020). Whey proteins and its derivatives: Bioactivity, functionality, and current applications. Dairy, 1 (3), 233-258.
Newbury, D. E., & Ritchie, N. W. M. (2014). Performing elemental microanalysis with high accuracy and high precision by scanning electron microscopy/silicon drift detector energy-dispersive X-ray spectrometry (SEM/SDD-EDS). Journal of Materials Science, 50 (2), 493-518.
Ribeiro-Santos, R., Andrade, M., Melo, N. R. de, & Sanches-Silva, A. (2017). Use of essential oils in active food packaging: Recent advances and future trends. Trends in Food Science and Technology, 61, 132-140.
Septiano, A. F., Susilo, S., & Setyaningsih, N. E. (2021). Analisis citra hasil Scanning Electron Microscopy Energy Dispersive X-Ray (SEM EDX) komposit resin timbal dengan metode Contrast to Noise Ratio (CNR). Indonesian Journal of Mathematics and Natural Sciences, 44 (2), 81-85.
Singh, A. K., Kim, J. Y., & Lee, Y. S. (2022). Phenolic compounds in active packaging and edible films/coatings: natural bioactive molecules and novel packaging ingredients. Molecules, 27 (21), 7513.
Totosaus, A., Godoy, I., & Ariza-Ortega, T. J. (2022). Physical, barrier, and thermal properties characterization of edible films from composite mixtures of starch and starch derivatives. The Annals of the University Dunarea de Jos of Galati, Fascicle VI: Food Technology, 46 (2), 175-187.
Tyuftin, A. A., Wang, L., Auty, M. A. E., & Kerry, J. P. (2020). Development and assessment of duplex and triplex laminated edible films using whey protein isolate, gelatin and sodium alginate. International Journal of Molecular Sciences, 21 (7), 2486.
Yiğit, A., Bielska, P., Cais-Sokolińska, D., & Samur, G. (2023). Whey proteins as a functional food: Health effects, functional properties, and applications in food. In Journal of the American Nutrition Association, 42 (8), 758-768.